Expression change of inflammatory cytokines and their receptors following Ang1 treatment.

Human neutrophils from at least 3 different individuals were treated with PBS, Ang1 or Ang2 (Table 2) at 10−8 M for 90 minutes. RT-qPCR array analyses were performed to assess expression change of 84 genes involved in the inflammatory response. Each gene from angiopoietin-treated neutrophils was compared to PBS-treated neutrophils and the data expressed as fold change. Negative and positive values denote a decrease and increase in mRNA expression, respectively. Differences were considered significant at Fold ≥ 4 and p<0.05. Housekeeping genes are denoted by an asterisk (*). Members of the IL-1 family that satisfied both requirements were considered significantly upregulated by Ang1, and are shaded in grey.