Identification of GmMYB176 interactome in soybean by Co-immunoprecipitation and LC-MS/MS

GmMYB176 is an R1 MYB transcription factor that regulates multiple genes in the isoflavonoid biosynthetic pathway thereby affecting isoflavonoid level in soybean roots. While GmMYB176 is important for isoflavonoid synthesis, it is not sufficient for the function and requires additional cofactor (s). The aim of this study was to identify GmMYB176 interactome for the regulation of isoflavonoid biosynthesis in soybean. GmMYB176 interactome was identified by Co-Immunoprecipitation using two YFP-tagged proteins baits, N’-GmMYB176-YFP-C’ and N’-YFP-GmMYB176-C’. GmMYB176 interacting proteins were identified by LC-MS/MS.