Fluorescence microscopy, image analysis and quantitative PCR data underlying the publication “Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting”

Spatial regulation of angiogenesis is important for the generation of functional engineered vasculature and the Notch ligands Jag1 and Dll4 are known to regulate endothelial sprouting. In this project, <em>in vitro </em>and <em>in silico </em>models were used to analyze the ability of micropatterned Jag1 and Dll4 to spatially control the sprouting process. Dll4 patterns, but not Jag1 patterns, were found to elicit spatial control and computational simulations suggest different timing of Notch activation by Jag1 and Dll4 to be the reason for their distinct effects. The more potent spatial control of sprouting by Dll4 in comparison to Jag1 could be exploited as a tool in vascular engineering and regenerative medicine. <br> This dataset contains data from <em>in vitro</em> experiments on endothelial sprouting with human endothelial cells and micropatterned Jag1 and Dll4 ligands.

血管生成的空间调控对于功能性工程化血管的构建具有重要意义，而Notch配体Jag1与Dll4已被证实可调控内皮细胞出芽。本研究采用体外（in vitro）与计算机模拟（in silico）模型，分析微图案化Jag1与Dll4对内皮细胞出芽过程的空间调控能力。研究发现，仅Dll4图案可实现空间调控，而Jag1图案无此效果；计算机模拟结果显示，Jag1与Dll4介导的Notch激活时序不同，这是二者产生不同调控效果的原因。相较于Jag1，Dll4具备更强的内皮细胞出芽空间调控能力，这一特性可应用于血管工程与再生医学领域。
本数据集包含采用人源内皮细胞与微图案化Jag1、Dll4配体开展的内皮细胞出芽体外实验数据。