Transcriptomic analysis of RNA psi modification in colorectal cancer reveals an epitranscriptomic regulation in cellular senescence

To identify the senescence level of PUS enzymes on colorectal cancer cells, we established WIDR cell lines in which DKC1 has been knocked down by shRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of DKC1 knockdown WIDR cells as well as negative control shRNA group. Overall design: Compare negative control shRNA and DKC1 knockdown WIDR cancer cells using RNA-seq.