2G12 serum antibody concentrations following passive administration.
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The concentrations of transferred 2G12 in the serum of all experimental animals on the day of challenge (day 0) and during the following three weeks were determined by ELISA using three different formats. For each animal, the results from the different ELISA formats are shown in separate columns. The concentrations of 2G12 in the macaque sera were determined from the measurement of binding to monomeric JR-FL (gp120), to an immobilized synthetic oligomannose dendron conjugated to BSA [21] (Man4D), and to a highly specific anti-idiotype-2G12 antibody (MIgG1 L13) [23] (anti-id). In all formats, a dilution series of serum was compared to a 2G12 standard curve and the concentration determined using a nonlinear regression curve fit analysis performed in GraphPad Prism Software for Mac, Version 5.0a.
创建时间:
2015-12-02



