Transcriptomic analysis of sturgeon embryos in response to chemically induced DNA damage

In this study we show that the exposure to genotoxicants (10 nM camptothecin and 20 µM Olaparib) affects embryo development in a stage-dependent manner with early stages being the most sensitive to DNA damage. The transcriptomic and proteomic patterns changed significantly after exposure to genotoxicants compared to the control. Camptothecin and Olaparib downregulated metabolic pathways and developmental processes. Additionally, the exposure to genotoxicants led to upregulation of cell proliferation, neural precursor cell proliferation, generation of neurons, taxis and locomotion. Protein patterns were altered as well by DNA damage. The results of this study indicate that there is a strong correlation between phenotype formation and changes in transcriptomic and proteomic profiles. Overall design: We analyzed the effect of the genotoxins, CPT (camptothecin) and Ola (olaparib) on the transcriptome of the Acipenser ruthenus embryos during the sxposure period from 2 to 24 hpf (blastula); 24 to 48 hpf (gastrulation) and 48 to 72 hpf (neurulation).