Antioxidant and antibacterial activities of the Bioactive compounds from the leaves of Ferulago trifida Boiss.

Isolation of compounds from methanol extract of the Ferulago trifida Boiss. leaves led to the identification of rutin (1), narcissin (2), heraclenol (3), xanthotoxin (4), imperatorin (5), bergapten (6), prantschimgin (7), umbelliferone (8), isogosferol (9), isopimpinellin (10), oxyimperatorin (11). Antibacterial effect of isolated compounds was studied and umbelliferone and isogosferol showed potent antibacterial activity on S. epidermidis (IZ; 15 mm, MIC; 500 μg mL−1) and S. dysenteriae (IZ; 16 mm, MIC; 500 μg mL−1), respectively. Antioxidant activity of methanol and chloroform fractions of leaves was investigated through 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) tests. The results showed mild antioxidant effect in DPPH test for methanol (IC50: 81.1) and chloroform (IC50: 125.2) extracts and weak antioxidant activity of methanol (67.1 ± 2.9 mmol FSE/100 g) and chloroform (77.4 ± 4.1 mmol FSE/100 g) based on FRAP test compared with BHT (267.2 ± 4.2 mmol FSE/100 g). Keywords: Ferulago trifida Boiss.; Apiaceae; coumarin; flavonoid; antioxidant; antibacterial