Cryo soft X-ray tomography of 5 microns thick slab extracted from C. elegans anterior region

Samples of C. elegans larvae of strain DM8005 were high pressure frozen using the waffle method. Multiple roughly 5 micron thick slabs spanning an L2 larva were extracted using the Serial Lift-Out technique, and placed on rectangular mesh grids. Soft X-ray tomography was performed on individual slabs using the SXT-100 soft x-ray microscope at SiriusXT. The data reveals label free internal structure of fully hydrated C. elegans tissue, annotating the worm volume and adding context to cryo electron tomography data. SXT data was acquired from -50° to +53° in 1° steps and reconstructed using Weighted Back Projection. A 32 nm objective zone plate was used. Data pixel size is 29 nm.