WAIS Divide Biological Data from J. Priscu and C. Foreman, Montana State University

Data from J. Priscu and C. Foreman, Montana State University. WAIS Divide core (WDC05Q, Stick D). Ice core samples were decontaminated following (Christner et al., 2005 Icarus, 174: 572-584) and handled in a class 100 environmental chamber. Meltwater was pre-filtered (30um) and gently sonicated prior to staining biotic particles with 5uM SYTO 60 (Invitrogen) for 5 min. Counts of biotic and abiotic particles were obtained using a Microcyte flow cytometer. Bacterial Productivity was determined via the incorporation of tritiated thymidine (TdR) into DNA at 6C following method of Fuhrman and Azam 1982 Marine Biology, 66: 109-120. The dissolved organic carbon (DOC) concentration of the ice core samples was obtained using a Shimadzu TOC 5000, see Christner et al., 2006 Limnology and Oceanography, 51(6): 2485-2501 for explanation of methods.