Bioinformatic identification of potential MicroRNA-Like Small RNAs in the edible mushroom Agaricus bisporus and experimental approach for their validation

Agaricus bisporus (the white-button mushroom, A. bisporus) is one of the most consumed mushroom worldwide and its genome was completely sequenced (H97 variety) , no information was found so far describing the presence of sRNAs in its edible parts (fruiting body and stipe). Therefore, this work was aimed to identify putative milRNAs from A. bisporus using NGS (next generation sequencing) and bioinformatics tools followed by an experimental approach with RTq-PCR to validate theoretical milRNAs candidates.