RNA-Seq profiling of Sirt1 deficient mouse oocytes to investigate Sirt1 role in maternal-zygotic transition

Sirtuin-1 (Sirt1), a NAD+-dependent histone deacetylase, exhibits several properties of a versatile driver of maternal-zygotic transition, due to its epigenetic and non-epigenetic substrates. The study was aimed at the dynamics of Sirt1 in early embryos and the contribution to maternal-zygotic transition. A conditional Sirt1-deficient knock-out mouse model was used. Females were hormonally stimulated and used as oocyte donors. oocytes were parthenogenetically activated and Sirt1-/- two-cell embryos were used for transcriptomic analysis.沉默信息调控因子1（Sirtuin-1，Sirt1）是一类烟酰胺腺嘌呤二核苷酸（nicotinamide adenine dinucleotide，NAD+）依赖的组蛋白去乙酰化酶，因其可靶向表观遗传与非表观遗传两类底物，具备作为母源-合子转换（maternal-zygotic transition）多效调控因子的多项特性。本研究旨在探究早期胚胎中Sirt1的动态变化及其对母源-合子转换的调控贡献。实验采用条件性Sirt1基因敲除小鼠模型，通过激素刺激雌性小鼠以获取卵母细胞供体，所获卵母细胞经孤雌激活后，以Sirt1纯合敲除的两细胞期胚胎为材料开展转录组学分析。