FibroTUG platforms: Time-lapse microscopy dataset of engineered cardiac microbundles

This dataset is a \"part II\" extension of the \"Engineered cardiac microbundle time-lapse microscopy image dataset\" and contains 808 experimental time-lapse image sequences of beating hiPSC-based cardiac microbundles using FibroTUG platforms [1] (\"Type2\"). In the \"part I\" extension, we include 732 experimental time-lapse image sequences of beating hiPSC-based cardiac microbundles using microbundle strain gauge platforms [2] (\"Type1\"). References: [1] DePalma, S. J., Davidson, C. D., Stis, A. E., Helms, A. S., & Baker, B. M. (2021). Microenvironmental determinants of organized iPSC-cardiomyocyte tissues on synthetic fibrous matrices. Biomaterials science, 9(1), 93-107. [2] Zhang, K., Cloonan, P. E., Sundaram, S., Liu, F., Das, S. L., Ewoldt, J. K., ... & Chen, C. S. (2021). Plakophilin-2 truncating variants impair cardiac contractility by disrupting sarcomere stability and organization. Science Advances, 7(42), eabh3995., In brief, experimental platforms of \"Type 2\" consist of electrospun fiber matrices suspended between a pair of PDMS cantilevers fabricated by soft lithography. Differentiated and purified iPSC-CMs were patterned onto the functionalized matrices using microfabricated seeding masks. After 3–21 days of seeding, time-lapse videos of the microbundle’s spontaneous contractions were acquired. The 808 examples included in this dataset were grown under 21 different tissue culture conditions, as detailed in the \"Type2_experimental_conditions.pdf\" document. The document \"Type2_common_experimental_metadata.pdf\" summarizes the common experimental conditions. Usage notes As previously mentioned, we include 808 examples of \"Type 2\" tissue. We share the raw videos in \".tif\" format and organize them based on: 1) platform type (\"Type 2\"), 2) experimental conditions under which the microbundles were grown (\"condition_000\", \"condition_001\",...), and 3) the batch of differentiated CMs (\"batch_000\", \"batch_0..., , # FibroTUG platforms: Time-lapse microscopy dataset of engineered cardiac microbundles [https://doi.org/10.5061/dryad.3r2280gqd](https://doi.org/10.5061/dryad.3r2280gqd) This dataset is a \"part II\" extension of the [`Engineered cardiac microbundle time-lapse microscopy image dataset`](https://doi.org/10.5061/dryad.5x69p8d8g) and contains 808 experimental time-lapse image sequences of beating hiPSC-based cardiac microbundles using FibroTUG platforms `[1]` (\"Type2\"). In \"part I\" extension, we include 732 experimental time-lapse image sequences of beating hiPSC-based cardiac microbundles using microbundle strain gauge platforms `[2]` (\"Type1\"). ## Description of the data and file structure We include here 808 examples of \"Type 2\" tissue. We share the raw videos in \".tif\" format and organize them based on: `1)` platform type (\"Type 2\"), `2)` experimental conditions under which the microbundles were grown (\"condition_000\", \"condition_001\",...), and `3)` the batch of differentiated CMs ...