RNA-sequencing of mouse double-negative developing thymocytes [WT and Duxbl[ind]xpTa[Cre]]

We performed a transcriptome comparison of double-negative developing thymocytes from the DN3-4 population, from mice overexpressing the transcription factor Duxbl and wild type mice Double-negative developing thymocytes from both WT and Duxbl[ind]xpTα[Cre] mice were gated for CD4-, CD8-, CD3-, B220-, CD25int, CD44low and CD117low expression, which define the DN3-4 stage of thymocyte development. The experiment was performed in four replicates, giving a total of 8 samples.