Conformationally Correct Expression of Membrane-Anchored Toxoplasma gondii SAG1 in the Primitive Protozoan Giardia duodenalis

To explore the possibility of expressing membrane-anchored exodomains of heterologous surface antigens in Giardia, a chimeric construct containing the Toxoplasma gondii SAG1 gene was made. The Giardia system is shown here to provide a means of generating correctly folded chimeric surface proteins in a native and unmodified form.