Populus nigra Transcriptome or Gene expression

Task 1.1 studies 4 Populus genotypes at three levels of drought stress under tightly controlled environmental conditions. Non-destructive measurements of photosynthesis, water use, and water status will be conducted in addition to NMR for water flux and innovative volatile measurements and leaf turgor. Leaves, developing xylem, and roots will be harvested for transcriptome (RNA-Seq), phosphoproteome and metabolome analyses, as well as for analyses of secondary metabolites, LOX, oxidative stress markers and phytohormones. Carbohydrates, carbon discrimination (13C) and amino acids will also be determined. Wood properties (anatomy, chemical composition, saccharification) will be measured.