Gut bacteria induce IgA expression in pituitary hormone-secreting cells during aging

Using the single-cell sequencing technology, the authors aim to make a construction of a single-cell cell atlas of young and old pituitary cells. Moreover, the sorted Ai6+ cells from pituitary of Igha-Cre; Ai6 were also analyzed by single-cell sequencing. Overall design: The sorted live young and old mouse pituitary cells, as well as sorted Igha-Cre; Ai6+ pituitary cells were captured and analyzed using the 10× Chromium platform.