Genome wide localization of the NuRD complex in Ewing sarcoma cells [CHIP-seq]

Chromatin immunoprecipitation followed by DNA sequencing of 4 NuRD complex subunits as well as for the fusion transcription factor EWS-FLI1 and relevant histone marks in Ewing sarcoma cells were performed to characterize the binding sites of the chromatin remodeling complex in relation to the fusion oncoprotein. Chromatin immunoprecipitation followed by DNA sequencing for FLI1, the NuRD subunits: CHD4, HDAC2, MTA2, and RBBP4, as well as for the histone marks H3K27ac, H3K27me3, H3K4me1 and H3K4me3 in Ewing sarcoma cells (SKNMC).