Validation Data Set For Automatic Blood Vessel Segmentation In Colorectal Cancer Histology (Ihc)

<strong>Content</strong> This data set contains 100 histological image patches of 1000 * 1000 px size. The samples were immunostained for CD34 (3,3'-Diaminobenzidine, DAB [brown]) with hematoxylin (blue) counterstain. Furthermore, the data set contains a table of blood vessel counts in each image by three blinded observers as well as an automatic count with a method based on the following paper: Kather, Jakob Nikolas et al. "Continuous Representation Of Tumor Microvessel Density And Detection Of Angiogenic Hotspots In Histological Whole-Slide Images". <em>Oncotarget</em> 6.22 (2015): 19163-19176. http://dx.doi.org/10.18632/oncotarget.4383 <strong>Image format</strong> All images are RGB, 0.50 µm per pixel, digitized with an Aperio ScanScope (Aperio/Leica biosystems), magnification 20x. Histological samples are fully anonymized images of formalin-fixed paraffin-embedded human colorectal adenocarcinomas (primary tumors and liver metastases) from our pathology archive (Institute of Pathology, University Medical Center Mannheim, Heidelberg University, Mannheim, Germany). <strong>Ethics statement</strong> All experiments were approved by the institutional ethics board (medical ethics board II, University Medical Center Mannheim, Heidelberg University, Germany; approval 2015-868R-MA). The institutional ethics board waived the need for informed consent for this retrospective analysis of anonymized samples. All experiments were carried out in accordance with the Declaration of Helsinki. <strong>Contact</strong> For questions, please contact:<br> Dr. Jakob Nikolas Kather<br> http://orcid.org/0000-0002-3730-5348<br> ResearcherID: D-4279-2015