RNA-Seq data of the spider mite sub-family Tetranychinae (Acari: Tetranychidae)

In this study, we developed a bioinformatics method using a k-mer counting algorithm for extracting species-specific primer candidate sequences from RNA-Seq data sets of 19 species of spider mites (Animalia, Arthropoda, Chelicerata, Arachnida, Acari, Tetranychidae). As a result of the k-mer analysis, 257-48,621 species-specific primer candidates per species were extracted. Then, 45 PCR primers were designed to be paired with species-specific primer candidates.