Liver transcriptome of Psoraleae Fructus decoction induced liver damage in mice

Psoraleae Fructus can cause liver damage, we study its effect on liver transcription,According to the sequence data, a total 6689 mRNAs were identified. Compared with control group, 1814 differential mRNAs were identified in the SPHD group, including 939 upregulated mRNAs and 875 downregulated mRNAs. Top 20 pathways are shown in the result, including “Metabolic pathways”, “NOD−like receptor signaling pathway”, “PI3K−Akt signaling pathway”, “Drug metabolism − other enzymes”, and “Estrogen signaling pathway”. To understand the functions of differential mRNAs, the target genes of differential mRNAs were predicted and were mapped to terms in the GO database and compared with the background (Figure 5C). The GO has 3 ontologies, which are “biological process”, “cellular component”, and “molecular function”. In the “biological process” part, most genes were related to “immune system process”, “oxidation−reduction process”, and “transport”. In the “cellular component” part, most genes were related to the “ribosomal part”, “mitochondrial part” and “membrane part”. In the “molecular function” part, most genes were related with “structural constituent of ribosome part”, “protein binding ” and “poly(A) RNA binding”. The high-dose Psoraleae Fructus decoction group was compared with the control group, with four in each groups.