基于MNP标记的暗褐脉柄牛肝菌菌株鉴定与群体遗传研究

Phlebopus portentosus, the first successfully domesticated and cultivated bolete species, has undergone rapid industrial expansion in recent years, highlighting the growing need for accurate strain identification and germplasm protection. In this study, whole-genome resequencing was performed on 66 strains of P. portentosus collected from various regions, resulting in the construction of a database of multiple nucleotide polymorphism (MNP) markers containing 306 loci. Population structure and kinship analyses based on both single nucleotide polymorphisms (SNPs) and MNPs consistently grouped the strains into two distinct genetic clusters. The MNP markers exhibited high resolution and stability across successive subculturing generations, enabling precise strain discrimination. Clonal identification criteria were established using replicate sequencing data of the same strains, with thresholds set at PI_HAT>0.95 and genetic distance (D)P. portentosus and validated the potential applications in genetic resource conservation and molecular breeding, providing scientific support for variety protection and germplasm innovation.