Reduced PGC-1α expression and mitochondrial biogenesis predisposes gliomas to radiation resistance

To investigate the role of mitochondrial metabolic pathways in glioma radioresistance, we established the radioresistant (RR) glioblastoma (GBM) cells in vitro and collected 2 self-pairs of primary and recurrent GBM samples. We then performed gene expression profiling analysis using data obtained from RNA-seq of GBM cells and tissue samples. Comparative gene expression profiling analysis, gene set enrichment analysis and gene set variation analysis of RNA-seq data were performed in GBM cells (U87, U251) versus their paired RR cells (U87-RR, U251-RR) and primary GBM tissue samples (Primary_1, Primary_2) versus their paired recurrent tissue samples (Recurrent_1, Recurreny_2), with 3 replicates for each type of GBM cell.