RNA-Seq analysis after overexpression of AtmiR165a in Arabidopsis Shoot Apical Meristem

Results from experiments done on knock-down of adaxial class III HD ZIP genes, using overexpression of miR165a, in shoot meristem show that Class III HD-ZIP genes act generally to repress the formation of new growth axes where they are expressed. This study explored the genes directly regualted by miRNAs as well as indirectly regulated by class III HD-ZIPs. Overall design: Comparative gene expression profiling analysis of RNA-seq data arabidopsis SAM of ap1cal mutant plants and ap1cal mutant plants expressing AtmiR165a under the Ubiquitin10 promoter.