Bioenergetic control of synaptic plasticity by astrocytes during acute stress

Aldh1l1CreERT2-RiboTag mice were used to purify and sequence astrocyte actively translated mRNAs from acute stress mouse models (20 minute forced swim) and non stressed controls. Four weeks after tamoxifen injection, somatosensory cortex was homogenized and RNA was purified from (i) cleared lysate as the input and control, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag. Stress mouse astrocyte transcriptomes were compared to non stressed.