Transcriptome analysis of RAW264.7 cells treated with CM-PEG-PLGA@DOX/R848 nanoparticles vs PBS control

RNA-seq analysis of RAW264.7 cells treated for 24 hours with CM-PEG-PLGA@DOX/R848 nanoparticles (equivalent DOX 2µg/ml) compared to PBS-treated controls. The study aims to identify transcriptomic changes induced by the nanomaterial treatment to to investigate the mechanism of immunomodulatory activity of CM-PEG-PLGA@DOX/R848 nanoparticles. Overall design: RAW264.7 cells were divided into two groups: experimental group treated with CM-PEG-PLGA@DOX/R848 nanoparticles and control group treated with PBS. After 24 hours of treatment, cells were harvested for RNA extraction and sequencing.