The overall effect of the various immunization strategies across ten different selected serum proteins on antibody yield, antisera titer, antibody specific activity, and assay sensitivity of different antibody combinations by ELISA.

1Geometric mean of assay sensitivity reported for homologous (self) and heterologous antibody combinations against native, and “within” immunization method.2Geometric mean of antibody yield for a 80-ml antiserum affinity purification run.3Geometric mean of antisera titers was intrapolated from a titration curve at 0.500 absorbance.