Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: Clinical Production Pilot (PPS). human metagenome

The HMP clinical production pilot study represents the pyrosequencing of 16S rRNA genes amplified from multiple body sites across a small number of subjects. Each clinical sample was sent to two HMP sequencing centers, each of which sequenced amplicons from at least one window, and maximum two (with default window: V3-V5, and additional window V1-V3). The goal of the study is to quantify the variation among replicates processed by various centers, to identify the optimal variable span of the 16S rRNA gene to target, and to gain a preliminary understanding of the diversity of microbial community structures among samples extracted from distinct body sites.