MeDIP-chip from livers, fibroblasts and sperm cells from adult cattle using anti-methylcytosine antibody.

Sperm cells are characterized by a unique epigenome, and an incorrect establishment of DNA methylation patterns during the differentiation of male germ cells into spermatozoa has been associated with male infertility in several species. While bull semen is widely used in artificial insemination, the literature describing DNA methylation in bovine sperm is still scarce. The purpose of this study is to compare the methylomes of sperm and somatic cell types in cattle using the MeDIP-chip technology (methylated DNA immunoprecipitation followed by microarray hybridation). Comparison of MeDIP DNA and input DNA with a dye-swap technical repetition for every sample; 11 samples in total, including 4 liver samples, 3 fibroblast samples and 4 sperm samples.