btuE expression alleviates oxidation of cytoplasmic proteins.
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Protein oxidation was determined in wild type, pBAD/btuE, ΔbtuE and ΔbtuE pBAD/btuE cells by the chemical protein carbonyl assay described in Methods. Total protein present in extracts of cells grown in the presence of 0.2% arabinose and exposed for 30 min to K2TeO3 (0.5 µg ml−1) or H2O2 (100 µM) were reacted with 2,4-dinitrophenylhydrazine, and the specific carbonyl absorbance was read at 370 nm. Values represent the mean of three independent experiments ± SD.
创建时间：
2015-12-02
