Cellular viability and ultrastructure of the long-term limbal cultures.

Tests were performed using CellTiter-Glo® Luminescent assay (A) and Trypan blue exclusion test (B). No significant difference could be observed in the cellular viability between the long- and short-term cultures, based on the ATP level measurements (n = 4). Long-term cultures represented an overall >90% viability (92.76±0.72%, n = 4), when tested by trypan blue exclusion. The ultrastructure of the 3D sheets is shown in (C): left image showing the stratified arrangement of the cells (bar: 20μm); right image showing cell-to-cell-interactions and formation of a desmosome at the center (bar: 1μm).