Dataset for: Acyl-CoA Binding Domain-Containing Protein 3 Functions as a Scaffold to Organize the Golgi Stacking Proteins and a Rab33b-GAP

Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA Binding Domain Containing 3 (ACBD3) is likely to be a novel binding partner of Golgin45. ACBD3 interacts with Golgin45 via its GOLD domain, and its co-expression significantly increases Golgin45 targeting to the Golgi. Further, ACBD3 recruits TBC1D22, a Rab33b GTPase Activating Protein (GAP), to a large multi-protein complex, containing Golgin45 and GRASP55. These results suggest that ACBD3 may provide a scaffolding to organize the Golgi stacking proteins and a Rab33b-GAP at the medial-Golgi.