Expression analyses of chordoma specific and candidate genes in MUG-Chor1 cells.

RT-qPCR was done on AB7900 TaqMan (Applied Biosystems; Foster City, CA). GAPDH and ACTB were used for normalization. Normalization and statistical analysis was done with GenEx Professional (MultiD Analysis; Version 5.3.5.6). All non-template controls were undetermined (Cq>45) except for GAPDH showing two replicates with Cq values >37 and VIM yielding one replicate at Cq = 27. Cut-off for multiple testing (ALG11, UCHL3, TMEM144 and PPP2CB) was p = 0.01274.acalculated as mean values from quadruplicate or triplicate (in case the Cq value could not be defined) biological samples.bCq values were normalized to GAPDH and ACTB (ΔCq). Differential expression (ΔΔCq) is given as positive (up-regulated in large cells) or negative (down-regulated in large cells) fold change ( = 2ΔΔCq).cCq values w/o outlier. Outliers were identified by means of Grubbs’ outlier test.