Unveiling the optimal parameters for cellulolytic characteristics of Talaromyces verruculosus SGMNPf3 and its secretory enzymes

AIMS: Elucidation of different physico-chemical parameters and the secretory enzymes released by Talaromyces verruculosus SGMNPf3 during cellulosic biomass degradation.
METHODS AND RESULTS: We determined the optimal pH, temperature and time course parameters for the efficient degradation of different natural and commercial cellulosic substrates by T. verruculosus SGMNPf3, previously isolated from a forest soil. The optimal growth of the fungus and production of its cellulases were obtained when the culture condition was maintained at pH 3·3 and temperature 30°C. Activity of the crude cellulases was maximum at 60°C. Activity of cellulase enzymes produced on natural cellulose substrates was higher than that on commercial cellulose substrates. A continuous increase in cellulase activity at different time points indicated no apparent end product inhibition. This might be attributed to the high individual cellulases, notably β-glucosidase (316·1 μmol g(-1) ) production. Zymogram of extracel...