File S1 - How Do Haloarchaea Synthesize Aromatic Amino Acids?

Supporting files. Figure S1, Southern blot analysis of WT, stopEO1472F (A) and InsOE1475F (B). A, Lane 1: size marker (in bp), lane2: SacI digested DNA from WT, lane 3: SacI digested DNA from StopOE1472F. B, Lane 1: size marker (in bp), lane2: NotI digested DNA from WT, lane 3: NotI digested DNA from InsOE1472F. Figure S2, AroAA regulated CHY and HY ORFs that are closely adjacent, and possibly organized in operons. Arrows show the relative positions and orientations of ORFs, but are not drawn to scale. The fold regulations are indicated below. *, **, represent ORF overlaps of 10 bp and 3 bp, respectively. The ORFs shown in panels A-D are described in Table 1. The ABC transporter ORFs shown in panel E is described in Table S2 in File S1. Table S1, Formation of 3-dehydroshikimate (DHS) from 3-dehydroquinate (DHQ) under different conditions. Table S2, Masses detected by LC-MS after derivatization with NBHA. Table S3, Expression of AroAA-related genes and transport-related genes in H. salinarum R1 cells grown in synthetic medium without AroAA relative to synthetic medium with AroAA. Table S4, Strains used in this study. Table S5, The composition of the chemically defined medium, pH = 7.0. Table S6, Plasmids used in this study. Table S7, List of oligonucleotides used in this study. (DOCX)