PLayer: A Plug-and-Play Embedded Neural System to Boost Neural Organoid 3D Reconstruction

This dataset supports the study titled "PLayer: A Plug-and-Play Embedded Neural System to Boost Neural Organoid 3D Reconstruction." It comprises a total of 539 high-resolution images, each with dimensions of 2048x2048 pixels. The image collection took place roughly over one month. We cultured the neural organoids ourselves based on the STEMdiff™ Cerebral Organoid Kit (STEMCELL Technologies Catalog #08570), aged 19, 34, 71, and 112 days, and the STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (STEMCELL Technologies Catalog #08620), aged 82 days. All organoids were collected on the same day, rinsed twice with PBS to remove any residual medium, and subsequently fixed with 4.0% (w/v) PFA at 4°C overnight before processing.