A BRD4-mediated elongation control point primes transcribing RNA polymerase II for 3'-processing and termination [NET-seq]

In this study, we reveal that BRD4 underlies a general 5'-elongation checkpoint that primes transcribing RNA polymerase II for 3'-RNA processing and transcription termination. BRD4-specific degradation impairs Pol II pause release, induces massive readthrough transcription, and RNA cleavage defects. Acute loss of BRD4 disrupts the recruitment of 3'-RNA processing factors. SI-NET-seq measurements for indicated time points upon DMSO (control), dTAG7 (BRD4 degradation) and dBET6 (pan-BET degradation) treatment in MOLT4 and K562 dTAG-BRD4 cells (two biological replicate measurements for each condition).