BIV Mekata flie1-10.zip

File.1: The list of 5 candidate of BIV primer and probe sets designed by the PrimerQuest Tool (https://sg.idtdna.com/pages/tools/primerquest). File 2-6: Results of melting curve analysis of 5 candidate primer sets. The positive and negative controls were the genome extracted from BIV strain R-29 and bovine whole blood, respectively. Nonspecific PCR products were identified in sets 2 and 5.File 7: Results of two sets of primers and probes (set1 and 3) attempting to detect BIV. The positive and negative controls were the genome extracted from BIV strain R-29 and bovine whole blood, respectively. Set1, which has a fast rise and strong fluorescence intensity, was used as the appropriate primer and probe.File 8: The detailed data of Figure 1 in the manuscript. We investigated whether the primers and probes developed for BIV detection affected the quantification of BLV by real-time PCR. Some of the data were removed or modified from the raw data due to the inclusion of individual cattle information and personal information of the farmers.File9: The detailed data of Table 2 in the manuscript. We examined the sensitivity of the real-time and nested PCR to detect BIV.File10_The data are a list of samples from which the epidemiology of BIV was studied. Some data were removed or modified from the raw data due to the inclusion of individual cattle information and personal information of farmers.