H3K27ac Hi-ChIP analysis in Jurkat and RPMI-8402 T-ALL cells

H3K27ac Hi-ChIP analysis was performed in Jurkat and RPMI-8402 stably expressing dCAR9-KRAB after treatment with DMSO (Control) or Doxycycline to induce sgRNA expression to block MYCN enhnacer region Overall design: Cells were treated with DMSO or doxycycline for 48 hours. The cells were subsequently collected and crosslinked with formaldehyde for 10 min. Hi-ChIP was performed using an antibody against H3K27ac (Abcam: ab4729). Cells were first lysed and then incubated with antibody against H3K27ac histone mark. DNA was enriched by chromatin-immunoprecipitation (ChIP), tagmented with Tn5, subjected for library construction, and sequenced by the DNBseq platform.