Additional file 2: Dataset S1. of Detection of pup odors by non-canonical adult vomeronasal neurons expressing an odorant receptor gene is influenced by sex and parenting status

Pairwise nucleotide sequence similarity for V2R receptors and probe sequence information. ‘V2R pairwise similarity matrix’ spreadsheet: Numbers in each cell represent nucleotide sequence similarity between a pair of V2R sequences in the region where in situ hybridization probes were designed. Similarity levels were calculated from genetic distance matrices produced from multiple alignments generated in MEGA used to construct phylogenetic trees in Additional file 6: Figure S5, according to the formula d = -3/4 ln (1-4/3 D), where d is the genetic distance and D is the fraction of nucleotide sites that differ between the pair of sequences being compared. The receptors are grouped into subclades, shown on the left, according to similarity levels. Triangles of yellow cells indicate comparisons between pairs of V2R sequences in the same subclade. ‘Oligo sequences’ spreadsheet: Sequences of oligonucleotides used as primers to amplify specific regions in target genes to be used for probe synthesis. (XLSX 104 kb)