Regulation of cardiac transcription by thyroid hormone and Med13

Purpose: The objective of this study was to determine cardiac transcriptional pathways regulated in response to 1.) hypothyroidism and re-establishment of a euthyroid state and 2.) Med13-dependent cardiac transcriptional pathways regulated in response to hypothyroidism and re-establishment of a euthyroid state Methods: WT and Med13 cardiac-specific knockout mice (Med13cKO) were put on a normal chow or PTU diet at 8 weeks of age for a duration of 4 weeks. A third group was put on a PTU diet for 4 weeks followed by 3 daily injections of T3.研究目的：本研究旨在探究两类受调控的心脏转录通路：1. 甲状腺功能减退症及甲状腺功能正常状态重建所调控的心脏转录通路；2. 甲状腺功能减退症及甲状腺功能正常状态重建过程中依赖Med13的心脏转录调控通路。实验方法：将野生型（wild type, WT）小鼠及心脏特异性敲除Med13的小鼠（Med13 cardiac-specific knockout mice, Med13cKO）于8周龄时分别饲喂普通维持饲料或丙基硫氧嘧啶（propylthiouracil, PTU）饲料，持续4周；另设第三组小鼠，先饲喂PTU饲料4周，随后每日注射三碘甲状腺原氨酸（Triiodothyronine, T3），连续3天。