Structures of FN and of the scFv C6.

A) Model of the domain structure of a FN subunit. The three different types of repeats, the three sites of alternative splicing (ED-A, ED-B, IIICS) and the specificity of the mAb C6 for the interface between type III repeats B and 8 are shown. B) Sequence of the scFv C6; the linker between the VL and the VH is in red; the CDRs are framed. Amino acids involved in the interaction with C6 are in bold. C) Sequence of the FN type III repeats numbers 7 (blue), B (black) and 8 (red); the various loops between the beta sheets structures are framed. The amino acids involved in the interaction with C6 are in bold. Sequence from http://www.ncbi.nlm.gov.nuccore/47132556.