DNA methylation dynamics in primary human monocytes exposed to thyroid hormone ex vivo

Trained immunity is a phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to certain microbial components, altering their responses to future exposures. This study profiled the genome-wide DNA methylation profile of human monocytes exposed to thyroid hormone for 24 hours. Human primary monocytes were isolated using Ficoll and two rounds of Percoll gradient separation. Baseline monocytes were collected after 1 hour in culture (attached) and 24 hours samples were collected for media only, LPS only, Thyroid hormone (T3), or T3+LPS.