Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with O-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with O-cresomycin, mRNA, deacylated A-site tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.50A resolution Descriptor: (4S,5aS,8S,8aR)-4-(2-methylpropyl)-N-[(1S,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-trihydroxy-7-methyl-2,13-dioxabicyclo[7.3.1]tridec-5-en-8-yl]octahydro-2H-oxepino[2,3-c]pyrrole-8-carboxamide (non-preferred name), 16S Ribosomal RNA, 23S Ribosomal RNA, ... Authors: Aleksandrova, E.V, Wu, K.J.Y, Robinson, P.J, Benedetto, A.E, Yu, M, Tresco, B.I.C, See, D.N.Y, Jiang, T, Ramkissoon, A, Dunand, C.F, Svetlov, M.S, Lee, J, Myers, A.G, Polikanov, Y.S. Deposit date: 2024-08-07 Release date: 2025-08-27 Last modified: 2026-03-04 Method: X-RAY DIFFRACTION (2.5 Å) Cite: Why Sulfur is Important in Lincosamide Antibiotics. Chem, 11, 2025

野生型嗜热栖热菌（Thermus thermophilus）70S核糖体与O-克列霉素（O-cresomycin）、信使RNA（mRNA）、脱酰基化A位点苯丙氨酸tRNA（deacylated A-site tRNAphe）、氨酰化P位点甲酰甲硫氨酸tRNA（aminoacylated P-site fMet-tRNAmet）以及脱酰基化E位点苯丙氨酸tRNA（deacylated E-site tRNAphe）结合的晶体结构，分辨率为2.50埃（Å）。
描述符：(4S,5aS,8S,8aR)-4-(2-甲基丙基)-N-[(1S,5Z,7R,8R,9R,10R,11S,12R)-10,11,12-三羟基-7-甲基-2,13-二氧杂双环[7.3.1]十三碳-5-烯-8-基]八氢-2H-氧杂环庚并[2,3-c]吡咯-8-甲酰胺（非惯用名称）、16S核糖体RNA（16S Ribosomal RNA）、23S核糖体RNA（23S Ribosomal RNA）……
作者：Aleksandrova, E.V.、Wu, K.J.Y.、Robinson, P.J.、Benedetto, A.E.、Yu, M.、Tresco, B.I.C.、See, D.N.Y.、Jiang, T.、Ramkissoon, A.、Dunand, C.F.、Svetlov, M.S.、Lee, J.、Myers, A.G.、Polikanov, Y.S.
提交日期：2024-08-07
发布日期：2025-08-27
最后修改日期：2026-03-04
实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率2.5埃（Å）
引用文献：《硫在林可酰胺类抗生素中的重要性》（Why Sulfur is Important in Lincosamide Antibiotics），《Chem》，第11卷，2025年。