IMPRINTS-CETSA, FUDR and FUR in MCF7 cells, 2h and 12h incubation

MCF7 cells were treated with floxuridine (FUDR), fluorouridine (FUR), or vehicle and assembled into an IMPRINTS-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC, 54ºC, and 57ºC) with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on the mass spectrometer.

This entry contains two IMPRINTS-CETSA datasets:
1. MCF7 incubated with Vehicle/FUDR/FUR for 2 hours
2. MCF7 incubated with Vehicle/FUDR/FUR for 12 hours

The labelling order of samples in the TMT10 channels for both datasets was as follows: 37C/47C/50C/52C/54C/57C samples: Biorep1_Vehicle (126), Biorep1_FUDR (127N), Biorep1_FUR (127C), Biorep2_Vehicle (128N), Biorep2_FUDR (128C), Biorep2_FUR (129N), Biorep3_Vehicle (129C), Biorep3_FUDR (130N), Biorep3_FUR (130C), mixed_control (131).

For additional details see the methods section of the associated publication.