Data from: Identification of candidate effector genes of Pratylenchus penetrans

Pratylenchus penetrans is one of the most important species among root lesion nematodes (RLNs) due to the detrimental and economic impact that it causes in a wide range of crops. Similar to other plant-parasitic nematodes (PPNs), P. penetrans harbors a significant number of secreted proteins that play key roles during parasitism. Here we combined spatially and temporally resolved next generation sequencing datasets of P. penetrans to select a list of candidate genes aimed at the identification of a panel of effector genes for this species. We determined the spatial expression of transcripts of 22 candidate effectors within the esophageal glands of P. penetrans by in situ hybridization. These comprised homologues of known effectors of other PPNs with diverse putative functions, as well as novel pioneer effectors specific to RLNs. It is noteworthy that five of the pioneer effectors encode extremely proline-rich proteins. We then combined in situ localization of effectors with available genomic data to identify a non-coding motif enriched in promoter regions of a subset of P. penetrans effectors, and thus a putative hallmark of spatial expression. Expression profiling analyses of a subset of candidate effectors confirmed their expression during plant infection. Our current results provide the most comprehensive panel of effectors found for RLNs. Considering the damage caused by P. penetrans, this information provides valuable data to elucidate the mode of parasitism of this nematode and offers useful suggestions regarding the potential use of P. penetrans-specific target effector genes to control this important pathogen. This article is protected by copyright. All rights reserved.短体线虫（*Pratylenchus penetrans*）是根腐线虫（root lesion nematodes, RLNs）类群中极具经济重要性的物种之一，可对多种作物造成严重危害与经济损失。与其他植物寄生线虫（plant-parasitic nematodes, PPNs）类似，短体线虫拥有大量在寄生过程中发挥关键作用的分泌蛋白。本研究整合了短体线虫的时空分辨率下一代测序数据集，以筛选出可用于构建该物种效应基因集的候选基因列表。通过原位杂交技术，本研究明确了22个候选效应因子在短体线虫食道腺中的转录本空间表达模式。这些候选效应因子既包含功能多样的其他植物寄生线虫已知效应因子的同源基因，也包含根腐线虫特异性的新型先锋效应因子。值得注意的是，其中5个先锋效应因子编码极富含脯氨酸的蛋白质。随后，本研究将效应因子的原位定位结果与现有基因组数据相结合，在部分短体线虫效应因子的启动子区域中鉴定出一段富集的非编码基序，由此推断其为空间表达的标志性特征。对部分候选效应因子的表达谱分析证实，它们在植物侵染过程中均有表达。本研究结果为根腐线虫类群提供了迄今为止最为全面的效应因子数据集。鉴于短体线虫所造成的危害，该研究结果可为阐明该线虫的寄生机制提供宝贵数据，并为开发针对短体线虫的特异性靶向效应基因防控策略提供有益参考。本文受版权保护，保留所有权利。