*Plating efficiencies were determined by taking ratios of number of transformants obtained with various SSB constructs in E. coli RDP317-1/pHYDEcoSSB by plating equal volumes from the same transformat
DH5 harbouring pHSG299 (*) or DH5 harbouring pHSG299-cam (†) was used as the donor strain. Transformation frequency (mean, n = 3) in each combination is presented in decimal ranges as follows: +++++,
The different B. subtilis strains studied were transformed using chromosomal DNA carrying a spectinomycin marker [35]. Each data point has been repeated at least 5 times which provides the standard de
The table shows the number of transformants obtained when 1 µg of the indicated DNA fragments was transformed into identical numbers of competent cells of the indicated strains. Each transformation mi
aAt the time of plating, the 168 and 168-pLK culture contained 1.3*109 viable cells/ml, the NCIB3610-pLK culture contained 0.9*109 viable cells/ml, and the JW49 contained 1.0*109 viable cells/ml. For
