mRNA sequencing of Nicotiana benthamiana

Experiments designed and performed by Martin H. Schattat and Jessica L. Erickson (Institute of Plant Physiology, Martin Luther University Halle-Wittenberg, Germany). Three transgenic N. benthamiana plants were grown on soil under 8h light (120µEm^{-2} ) and 16h dark at 20° C. The transgene, an expression cassette facilitating the expression of a stroma targeted eGFP, was introduced into N. benthamiana for the purpose of examining the plastids. A total of approximately 100mg of plant tissue was harvested from the 3rd and 4th leaves of six week old plants. The Qiagen RNeasy Plant Mini Kit was used to isolate total RNA from the three plants (three biological replicates), which was then sent to MWG Eurofins (Ebersberg, Germany) for RNA sequencing with Illumina HiSeq 2000 (v3.0 chemistry).