Bulk RNA-seq analysis of PDAC PANC-1 cells silenced with siRNA targeting myoferlin or treated with WJ460.

Myoferlin is a transmembrane protein, encoded by the MYOF gene, initially identified in myoblasts for its crucial role in membrane biology via its C2 domain. MYOF was overexpressed in PDAC and functional studies have revealed myoferlin's critical involvement in mitochondrial adaptation, metatstasis and ferroptosis. PANC-1 cells were transfected with irrelevant (GL3), myoferlin specific siRNA or treated with myoferlin-targetting compound (WJ460, 50nM). Cells were collected 48h after cell transfection/treatment and submitted to bulk RNA-seq.