Comparison of PRV-MdBio wild type viral transcriptomes derived from PK-15, C6, PC-12 and NRK cell lines in different time points of infection sequenced using direct cDNA and direct RNA sequencing. Long read transcriptome sequencing of wild type PRV on four different cell lines

Whole transcriptome of the wild-type PRV isolate (strain PRV-MdBio) was analyzed in different time points of infections. The virus was propagated on three different cell lines: porcine kidney epithelial cell line (PK-15), C6 cell line derived from a rat glial tumor, PC-12 cells derived from a pheochromocytoma of the rat adrenal medulla and NRK cells derived from rat kidney cells. Poly-A selected viral RNA was isolated in the following time points post-infection: 1h,2h,4h,6h,8h,12h in triplicates. Direct cDNA from each time points and dRNA from a mixture of time points was sequenced on Oxford Nanopore MinION sequencer device. The comparative transcriptome analysis enables temporal differentiation of viral and host cell gene expression.