BGI_F21FTSCCKF9401_MUSqodbT.rar

Over all 15 oocytes in each group were prepared for single-cell RNA sequencing analysis. Three parallel experiments were conducted for each group. After 9 hours of culturing in three group (control, 4 μM rotenone, 4 μM rotenone plus 10-9 μM melatonin), MI oocytes from were collected. All samples were individually infiltrated in 0.2 mL PCR tubes containing 4 μL lysis buffer and stored at -80℃ in RNA extraction buffer. Total RNA was extracted from each sample according to the manufacturer’s protocol. cDNA library construction and RNA-Seq analysis were performed by Beijing Genomics Institution (BGI, China). Gene with FPKM >1 were identified for differential expression analysis. Differentially expressed genes (DEGs) were screened with the NOISeq package according to “fold change” >2 between two groups. Gene Ontology (GO) analysis was performed using the GO database (http://www.geneontology.org/). An adjusted P-value <0.05 indicated significant gene enrichment. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the enrichment of DEGs was performed using the KEGG pathway database (http://www.genome.jp/kegg/). An adjusted P-value <0.05 indicated significant gene enrichment in the pathways.